Method Development: The Antioxidant and Antifungal Activity of the Isolated Component from the Ethanolic Extract of Tecoma stans Leaves Using Flash Chromatography

Tecoma stans is an ornamental perennial tropical and subtropical plant belonging to the Bignoniaceae family with green leaves yellow attractive fragrance flowers commonly known as trumpetbush or bells. The originated in areas of South America Mexico has been cultivated many countries such Egypt, Iraq, United Arab Emirates (UAE). T. found different parts UAE Ras Al Khaimah, Abu Dhabi, Dubai, where it can be seen public parks, side roads, home gardens. Flash Chromatography System used aspects drug discovery studies because its ability purify secondary metabolites from crude extracts. A method was developed using isolate three components ethanolic extract that showed vitro antioxidant activity. In evaluation activity isolated conducted 1,1-diphenyl-2-picryl hydrazyl 2, 2′-azinobis-3-ethylbenzothiazoline-6-sulfonic acid methods. Isolated A-4, A-3, A-2 had when compared ascorbic acid. Component A-3 DPPH ABTS methods; antifungal activities tested against Candida albicans; more than 80% inhibitions third dilution itraconazole nystatin positive controls. This rapid efficient flash chromatography for isolation purification component both activities.